Which of the following techniques is used for quantification of mRNA?

Q. Which of the following techniques is used for quantification of mRNA?

A) Northern blotting
B) Reverse transcriptase PCR
C) Real-time PCR
D) Gradient PCR

... Answer is C) Real-time PCR

The quantitative RT-PCR is a method used to quantify mRNA levels. This technique is particularly useful to quantitate expression levels of transcripts that are expressed at very low levels, such as GTs. Real-time RT-PCR has gained popularity not only in quantifying gene expression but to confirming differential expression of genes detected by microarray analysis. Real-time RT-PCR uses commer- cially available fluorescence-detecting thermal cyclers to simultaneously amplify specific DNA sequences and measure their concentration. This method is optimal for measuring DNA concentration over a wide dynamic range, its sensitivity is high, and many samples can be processed simultaneously.